3.25 in. x 4 in. Lantern Slides Original Magnification: x2,000 This study demonstrated directly that the enzyme and proenzyme composition of proteins in pancreatic juice collected from an indwelling catheter in the bovine pancreatic duct correlated exactly with the protein composition obtained from lysed purified bovine zymogen granules. Subsequent studies showed that pancreatic digestive enzymes and proenzymes were secreted from the pancreas in parallel. In other words, each granule must contain, on average, the same composition of secretory proteins. This hypothesis was later directly tested by immuno-EM localization of trypsinogen and other secretory proteins in all zymogen granules in the bovine pancreas.
Zymogen granule fraction
Jamieson, James D
transmission electron micrograph
Prints & Photographs
The use of this image may be subject to the copyright law of the United States (Title 17, United States Code) or to site license or other rights management terms and conditions. The person using the image is liable for any infringement Yale Community Only
Yale University School of Medicine, Department of Cell Biology
George E. Palade Electron Microscopy Slide Collection
See Greene, L. J., C.H. Hirs, and G.E. Palade. 1963. On the protein composition of bovine pancreatic zymogen granules. J. Biol. Chem. 238:2054-2070.
For more information about this resource, contact:
Harvey Cushing/John Hay Whitney Medical Library
333 Cedar Street
New Haven, CT 06520