Immune Cells Rat myocardium plasma cell

Creator:
Palade, George E
Published/Created:
New Haven, CT
Yale University School of Medicine
2/2/1970
Physical Description:
3.25 in. x 4 in.
Notes:
3.25 in. x 4 in. Lantern Slides
Original Magnification: x31,000
This study examined the intracellular secretory pathway for IgG using tritiated leucine to tag the protein backbone and tritiated galactose and glucosamine to tag the glycoprotein chains synthesized in the Golgi. EM autoradiography was used to follow the travels of IgG after synthesis. The results indicated that IgG (the major protein synthesized by these myeloma cells) moved from the RER to the Golgi for sugar addition to IgG and likely reached the cell surface via small smooth surfaced transport vesicles. Previously it had been suggested that IgG moved in soluble form through the cytoplasm and was secreted directly through the plasma membrane. No evidence was obtained for temporary storage in secretory granules.
Topics:
Immune Cells
Topics:
Jamieson, James D
Genre:
transmission electron micrograph
Format:
Image
Content Type:
Prints & Photographs
Rights:
The use of this image may be subject to the copyright law of the United States (Title 17, United States Code) or to site license or other rights management terms and conditions. The person using the image is liable for any infringement
Yale Community Only
Access Restrictions:
Public
Yale Collection:
Yale University School of Medicine, Department of Cell Biology
Digital Collection:
George E. Palade Electron Microscopy Slide Collection
Citation:
See Zagury, D., J.W. Uhr, J.D. Jamieson and G.E. Palade. 1970. Immunoglobulin synthesis and secretion: II. Radioautographic studies of sites of addition of carbohydrate moieties and intracellular transport. J. Cell Biol. 46:52-63.
OID:
15817904
PID:
digcoll:2795408