Techniques Whole mount of fibroblast: preparation for classic TEM Porter, Claude and Fullam

Creator:
Claude, A
Fullam, E.F
Porter, K
Published/Created:
New Haven, CT
Yale University School of Medicine
Physical Description:
3.25 in. x 4 in.
Notes:
3.25 in. x 4 in. Lantern Slides
Classic TEM. First description of the endoplasmic reticulum. Whole mount of unfixed, dried chick embryo fibroblast. The genius of their approach was to grow embryonic chick cells on a thin collodion film supported by a fine mesh copper grid. After the cells had grown and spread out thin processes, the preparation was air dried. They reasoned that the thin processes would allow electrons to penetrate in the electron microscope. The only microscope available to them was owned by the Interchemical Corp. and housed in the Empire State Building. Fullam was the technician. The electron microscope was a first generation RCA type B.
Topics:
Techniques
Topics:
Jamieson, James D
Genre:
transmission electron micrograph
Format:
Image
Content Type:
Prints & Photographs
Rights:
The use of this image may be subject to the copyright law of the United States (Title 17, United States Code) or to site license or other rights management terms and conditions. The person using the image is liable for any infringement
Yale Community Only
Access Restrictions:
Public
Yale Collection:
Yale University School of Medicine, Department of Cell Biology
Digital Collection:
George E. Palade Electron Microscopy Slide Collection
Citation:
See Porter, K.R., A. Claude and E.F. Fullam. 1945. Study of tissue culture cells by electron microscopy. J. Exp. Med. 81:233-246.
OID:
15817974
PID:
digcoll:2795391