Techniques Whole mount of fibroblast: preparation for classic TEM Porter, Claude and Fullam
Claude, A Fullam, E.F Porter, K
New Haven, CT Yale University School of Medicine
3.25 in. x 4 in.
3.25 in. x 4 in. Lantern Slides Classic TEM. First description of the endoplasmic reticulum. Whole mount of unfixed, dried chick embryo fibroblast. The genius of their approach was to grow embryonic chick cells on a thin collodion film supported by a fine mesh copper grid. After the cells had grown and spread out thin processes, the preparation was air dried. They reasoned that the thin processes would allow electrons to penetrate in the electron microscope. The only microscope available to them was owned by the Interchemical Corp. and housed in the Empire State Building. Fullam was the technician. The electron microscope was a first generation RCA type B.
Jamieson, James D
transmission electron micrograph
Prints & Photographs
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Yale University School of Medicine, Department of Cell Biology
George E. Palade Electron Microscopy Slide Collection
See Porter, K.R., A. Claude and E.F. Fullam. 1945. Study of tissue culture cells by electron microscopy. J. Exp. Med. 81:233-246.
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