New Haven, CT Yale University School of Medicine 11/11/1970
3.25 in. x 4 in.
3.25 in. x 4 in. Lantern Slides Original Magnification: x13,750 The authors took advantage of the fact that rats gavage-fed a large bolus of ethanol (single malt I hope) were observed to have their Golgi cisternae loaded with LDL particles. Since LDL particles are lighter than most other intracellular organelles, following homogenization of ethanol-fed rats, the Golgi fractions "floated" to the top of denser sucrose gradients during centrifugation. This allowed investigators to analyze the membrane properties of "pure" Golgi fractions and set the stage for development of fusion assays that led to an understanding of factors regulating the specificity of membrane fusion - the SNARE hypothesis.
Jamieson, James D
transmission electron micrograph
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Yale University School of Medicine, Department of Cell Biology
George E. Palade Electron Microscopy Slide Collection
See Ehrenreich, J.H., J.J.M. Bergeron, P. Siekevitz and G.E. Palade. 1973. Golgi fractions prepared from rat liver homogenates: I. Isolation procedure and morphological characterization. J. Cell. Biol. 59:45-72.
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